# SCTLD rapid microbiome characterization # Stony Coral Tissue Loss Disease in St. Thomas, US Virgin Islands, 2020 # PI: A, Apprill # version date: 2020-12-07 # ========================= Sample_ID NCBI_BioProject_accession_number NCBI_BioSample_accession_number Sample_type Sequencing_Strategy Sequencing_Instrument_model Sequencing_strategy_details Reef_Name Latitude Longitude Coral_species iSeq_run_number reef_type SCTLD_status SCTLD_duration_months reef_depth_m Temperature_C Salinity_psu pH sample_type Disease_Present Disease_Sample_Type Project_Colony_Code Lesion_Distribution Lesion_Start Lesion_Margin Disease_Colony_Sample_Distance_cm Coral_Clade DNA_extraction_method variable_region_16S Primer_Pair ------------------------- 163eco PRJNA672912 SAMN16582909 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD MCAV10 focal edge of colony recent mortality of lesions next to apparently healthy tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 164eco PRJNA672912 SAMN16582910 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD CNAT4 focal edge of colony bleaching band; healthy tissue paled nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 165eco PRJNA672912 SAMN16582911 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD MCAV7 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 166eco PRJNA672912 SAMN16582912 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD MCAV7 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 167eco PRJNA672912 SAMN16582913 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD MCAV10 focal edge of colony recent mortality of lesions next to apparently healthy tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 168eco PRJNA672912 SAMN16582914 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD MCAV9 multifocal both recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue; discoloration of healthy tissue (paling) nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 169eco PRJNA672912 SAMN16582915 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD MCAV9 multifocal both recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue; discoloration of healthy tissue (paling) 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 170eco PRJNA672912 SAMN16582916 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD MCAV8 multifocal edge of colony recent mortality of lesions next to apparently healthy tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 171eco PRJNA672912 SAMN16582917 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD MCAV8 multifocal edge of colony recent mortality of lesions next to apparently healthy tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 172eco PRJNA672912 SAMN16582918 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD CNAT4 focal edge of colony bleaching band; healthy tissue paled 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 173eco PRJNA672912 SAMN16582919 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD CNAT5 focal edge of colony bleaching band; healthy tissue paled 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 174eco PRJNA672912 SAMN16582920 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD CNAT5 focal edge of colony bleaching band; healthy tissue paled nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 175eco PRJNA672912 SAMN16582921 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HH MCAV13 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 177eco PRJNA672912 SAMN16582922 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD CNAT7 unknown unknown bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 178eco PRJNA672912 SAMN16582923 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HH MCAV14 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 179eco PRJNA672912 SAMN16582924 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD CNAT2 multifocal both bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 180eco PRJNA672912 SAMN16582925 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD CNAT1 focal middle of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 181eco PRJNA672912 SAMN16582926 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MMEA3 focal middle of colony bleaching band 3 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 182eco PRJNA672912 SAMN16582927 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MMEA3 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 183eco PRJNA672912 SAMN16582928 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MCAV2 multifocal both bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 184eco PRJNA672912 SAMN16582929 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MMEA1 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 185eco PRJNA672912 SAMN16582930 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HH MCAV4 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 186eco PRJNA672912 SAMN16582931 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD OFRA2 focal edge of colony apparently healthy tissue paled; bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 187eco PRJNA672912 SAMN16582932 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MMEA1 focal middle of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 188eco PRJNA672912 SAMN16582933 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HH MCAV5 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 189eco PRJNA672912 SAMN16582934 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HH MCAV6 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 190eco PRJNA672912 SAMN16582935 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MCAV3 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 191eco PRJNA672912 SAMN16582936 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD CNAT3 multifocal middle of colony bleaching band; patchy paling of healthy coral tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 192eco PRJNA672912 SAMN16582937 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MCAV3 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 193eco PRJNA672912 SAMN16582938 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MMEA5 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 194eco PRJNA672912 SAMN16582939 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MMEA2 focal unknown bleaching band 3 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 195eco PRJNA672912 SAMN16582940 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MCAV2 multifocal both bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 196eco PRJNA672912 SAMN16582941 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD OFRA3 multifocal edge of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 197eco PRJNA672912 SAMN16582942 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD CNAT1 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 198eco PRJNA672912 SAMN16582943 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD CNAT3 multifocal middle of colony bleaching band; patchy paling of healthy coral tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 199eco PRJNA672912 SAMN16582944 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run2_BPL20308-1024 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD OFRA1 unknown unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 200eco PRJNA672912 SAMN16582945 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD OFRA2 focal edge of colony apparently healthy tissue paled; bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 201eco PRJNA672912 SAMN16582946 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD CNAT2 multifocal both bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 202eco PRJNA672912 SAMN16582947 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MMEA2 focal unknown bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 203eco PRJNA672912 SAMN16582948 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD OFRA3 multifocal edge of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 204eco PRJNA672912 SAMN16582949 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HH MMEA4 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 205eco PRJNA672912 SAMN16582950 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD OFRA1 unknown unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 5 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 206eco PRJNA672912 SAMN16582951 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MCAV1 focal edge of colony recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 207eco PRJNA672912 SAMN16582952 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Seawater less than 5cm from coral surface y DD MCAV1 focal edge of colony recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 208eco PRJNA672912 SAMN16582953 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Seawater less than 5cm from coral surface n HD MMEA5 focal middle of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 268eco PRJNA672912 SAMN16582954 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD CNAT6 focal edge of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 270eco PRJNA672912 SAMN16582955 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HH MCAV12 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 271eco PRJNA672912 SAMN16582956 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD CNAT6 focal edge of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 272eco PRJNA672912 SAMN16582957 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD CNAT7 unknown unknown bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 273eco PRJNA672912 SAMN16582958 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface n HD CNAT8 multifocal edge of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 274eco PRJNA672912 SAMN16582959 near coral seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Seawater less than 5cm from coral surface y DD CNAT8 multifocal edge of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 81tiss PRJNA672912 SAMN16582960 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry y DD MCAV1 focal edge of colony recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 82tiss PRJNA672912 SAMN16582961 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry y DD MCAV3 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 83tiss PRJNA672912 SAMN16582962 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry y DD MCAV2 multifocal both bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 84tiss PRJNA672912 SAMN16582963 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry n HD MCAV2 multifocal both bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 85tiss PRJNA672912 SAMN16582964 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run2_BPL20308-1024 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry y DD OFRA1 unknown unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 86tiss PRJNA672912 SAMN16582965 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry n HD OFRA1 unknown unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 5 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 87tiss PRJNA672912 SAMN16582966 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry n HD MCAV3 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 88tiss PRJNA672912 SAMN16582967 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 14.2 26.88 35.98 7.98 Tissue Slurry n HD MCAV1 focal edge of colony recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 89tiss PRJNA672912 SAMN16582968 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD OFRA2 focal edge of colony apparently healthy tissue paled; bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 90tiss PRJNA672912 SAMN16582969 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD CNAT3 multifocal middle of colony bleaching band; patchy paling of healthy coral tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 91tiss PRJNA672912 SAMN16582970 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD OFRA3 multifocal edge of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 92tiss PRJNA672912 SAMN16582971 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD OFRA3 multifocal edge of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 93tiss PRJNA672912 SAMN16582972 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD CNAT3 multifocal middle of colony bleaching band; patchy paling of healthy coral tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 94tiss PRJNA672912 SAMN16582973 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD MMEA5 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 95tiss PRJNA672912 SAMN16582974 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HH MMEA4 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 97tiss PRJNA672912 SAMN16582975 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HH MCAV6 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 98tiss PRJNA672912 SAMN16582976 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 coral reef seawater Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry nd DD SW Ctrl 2 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 99tiss PRJNA672912 SAMN16582977 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD MMEA3 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 100tiss PRJNA672912 SAMN16582978 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 coral reef seawater Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry nd nd SW Ctrl 1 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 102tiss PRJNA672912 SAMN16582979 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD MMEA3 focal middle of colony bleaching band 3 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 103tiss PRJNA672912 SAMN16582980 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Orbicella franksi Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD OFRA2 focal edge of colony apparently healthy tissue paled; bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 104tiss PRJNA672912 SAMN16582981 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 coral reef seawater Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry nd nd SW Ctrl 3 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 105tiss PRJNA672912 SAMN16582982 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD MMEA5 focal middle of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 212tiss PRJNA672912 SAMN16582983 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD MCAV10 focal edge of colony recent mortality of lesions next to apparently healthy tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 214tiss PRJNA672912 SAMN16582984 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD MCAV8 multifocal edge of colony recent mortality of lesions next to apparently healthy tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 215tiss PRJNA672912 SAMN16582985 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry y DD CNAT4 focal edge of colony bleaching band; healthy tissue paled nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 217tiss PRJNA672912 SAMN16582986 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry y DD MCAV7 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 218tiss PRJNA672912 SAMN16582987 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 coral reef seawater Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry nd nd SW Ctrl 5 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 219tiss PRJNA672912 SAMN16582988 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD MCAV11 multifocal edge of colony recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 220tiss PRJNA672912 SAMN16582989 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD CNAT4 focal edge of colony bleaching band; healthy tissue paled 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 222tiss PRJNA672912 SAMN16582990 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry y DD MCAV8 multifocal edge of colony recent mortality of lesions next to apparently healthy tissue nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 224tiss PRJNA672912 SAMN16582991 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 coral reef seawater Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry nd nd SW Ctrl 4 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 225tis PRJNA672912 SAMN16582992 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry y DD MCAV9 multifocal both recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue; discoloration of healthy tissue (paling) nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 227tiss PRJNA672912 SAMN16582993 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 coral reef seawater Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry nd nd SW Ctrl 6 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 228tiss PRJNA672912 SAMN16582994 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD MCAV9 multifocal both recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue; discoloration of healthy tissue (paling) 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 229tiss PRJNA672912 SAMN16582995 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry y DD CNAT5 focal edge of colony bleaching band; healthy tissue paled nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 230tiss PRJNA672912 SAMN16582996 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run3_BPL20308-3425 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD MCAV7 multifocal unknown recent mortality of lesions next to apparently healthy tissue; some bleached-like tissue 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 232tiss PRJNA672912 SAMN16582997 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 12 11.1 26.98 36.04 8.09 Tissue Slurry n HD CNAT5 focal edge of colony bleaching band; healthy tissue paled 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 234tiss PRJNA672912 SAMN16582998 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry n HH MCAV14 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 235tiss PRJNA672912 SAMN16582999 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry n HD CNAT8 multifocal edge of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 248tiss PRJNA672912 SAMN16583000 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 coral reef seawater Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry nd nd SW Ctrl 9 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 250tiss PRJNA672912 SAMN16583001 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry n HD CNAT7 unknown unknown bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 251tiss PRJNA672912 SAMN16583002 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry n HD CNAT6 focal edge of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 253tiss PRJNA672912 SAMN16583003 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 coral reef seawater Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry nd nd SW Ctrl 7 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 256tiss PRJNA672912 SAMN16583004 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry y DD CNAT8 multifocal edge of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 259tiss PRJNA672912 SAMN16583005 reef seawater SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 coral reef seawater Run3_BPL20308-3425 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry nd nd SW Ctrl 8 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 262tiss PRJNA672912 SAMN16583006 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry y DD CNAT6 focal edge of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 264tiss PRJNA672912 SAMN16583007 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Black Point 18.3445 -64.98595 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 12 11.8 26.98 36.04 8.09 Tissue Slurry y DD CNAT7 unknown unknown bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 75Ltiss PRJNA672912 SAMN16583008 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HH MCAV5 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 76Ltiss PRJNA672912 SAMN16583009 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD MMEA2 focal unknown bleaching band 3 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 77Ltiss PRJNA672912 SAMN16583010 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD CNAT1 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 78Ltiss PRJNA672912 SAMN16583011 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD CNAT2 multifocal both bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 79Ltiss PRJNA672912 SAMN16583012 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Montastraea cavernosa Run2_BPL20308-1024 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HH MCAV4 nd nd nd nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 80Ltiss PRJNA672912 SAMN16583013 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD CNAT2 multifocal both bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 81Ltiss PRJNA672912 SAMN16583014 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Colpophyllia natans Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD CNAT1 focal middle of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 82Ltiss PRJNA672912 SAMN16583015 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry n HD MMEA1 focal middle of colony bleaching band 10 Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 83Ltiss PRJNA672912 SAMN16583016 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run3_BPL20308-3425 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD MMEA1 focal middle of colony bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R 84Ltiss PRJNA672912 SAMN16583017 surface tissue and mucus SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing Buck Island 18.27883 -64.89833 Meandrina meandrites Run1_BPC29622-1521 fringing epidemic 1 11.9 26.88 35.98 7.98 Tissue Slurry y DD MMEA2 focal unknown bleaching band nd Robust Qiagen Dneasy PowerBiofilm V4 515F 806R D1 PRJNA672912 SAMN16583018 DNA extraction from empty bead tube SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run1_BPC29622-1521 nd nd nd nd nd nd nd Tissue Slurry DNA extraction control (blank bead tube) nd nd nd nd nd nd nd nd Qiagen Dneasy PowerBiofilm V4 515F 806R D2 PRJNA672912 SAMN16583019 DNA extraction from empty bead tube SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run2_BPL20308-1024 nd nd nd nd nd nd nd Tissue Slurry DNA extraction control (blank bead tube) nd nd nd nd nd nd nd nd Qiagen Dneasy PowerBiofilm V4 515F 806R D3 PRJNA672912 SAMN16583020 DNA extraction from empty bead tube SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run3_BPL20308-3425 nd nd nd nd nd nd nd Tissue Slurry DNA extraction control (blank bead tube) nd nd nd nd nd nd nd nd Qiagen Dneasy PowerBiofilm V4 515F 806R D4 PRJNA672912 SAMN16583021 DNA extraction from unused seawater filter SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run1_BPC29622-1521 nd nd nd nd nd nd nd Seawater filter DNA extraction control (unused 0.22 um filter) nd nd nd nd nd nd nd nd Qiagen Dneasy PowerBiofilm V4 515F 806R D5 PRJNA672912 SAMN16583022 DNA extraction from unused seawater filter SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run2_BPL20308-1024 nd nd nd nd nd nd nd Seawater filter DNA extraction control (unused 0.22 um filter) nd nd nd nd nd nd nd nd Qiagen Dneasy PowerBiofilm V4 515F 806R D6 PRJNA672912 SAMN16583023 DNA extraction from unused seawater filter SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run3_BPL20308-3425 nd nd nd nd nd nd nd Seawater filter DNA extraction control (unused 0.22 um filter) nd nd nd nd nd nd nd nd Qiagen Dneasy PowerBiofilm V4 515F 806R H2Ocontrol-1 PRJNA672912 SAMN16583024 PCR grade water SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run1_BPC29622-1521 nd nd nd nd nd nd nd PCR negative control (sterile PCR-grade water) nd nd nd nd nd nd nd nd nd V4 515F 806R H2Ocontrol-2 PRJNA672912 SAMN16583025 PCR grade water SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run2_BPL20308-1024 nd nd nd nd nd nd nd PCR negative control (sterile PCR-grade water) nd nd nd nd nd nd nd nd nd V4 515F 806R H2Ocontrol-3 PRJNA672912 SAMN16583026 PCR grade water SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run3_BPL20308-3425 nd nd nd nd nd nd nd PCR negative control (sterile PCR-grade water) nd nd nd nd nd nd nd nd nd V4 515F 806R MockE-1 PRJNA672912 SAMN16583027 nd SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run1_BPC29622-1521 nd nd nd nd nd nd nd Microbial Mock Community B; Even (HMP-782D) nd nd nd nd nd nd nd nd nd V4 515F 806R MockE-2 PRJNA672912 SAMN16583028 nd SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run2_BPL20308-1024 nd nd nd nd nd nd nd Microbial Mock Community B; Even (HMP-782D) nd nd nd nd nd nd nd nd nd V4 515F 806R MockE-3 PRJNA672912 SAMN16583029 nd SSU rRNA amplicon lllumina iSeq 100 System 515FY/806RB primers amplified the V4 region of the SSU rRNA gene; and sequencing was conducted using 2x150bp sequencing nd 18.3704 -64.9804 nd Run3_BPL20308-3425 nd nd nd nd nd nd nd Microbial Mock Community B; Even (HMP-782D) nd nd nd nd nd nd nd nd nd V4 515F 806R